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目的:观察大鼠癫痫发作后海马齿状回细胞因子IL-1(IL-1R、IL-1β、IL-1ra)mRNA的表达变化,探讨IL-1在癫痫发作中的作用。方法:大鼠随机分为对照组和实验组。实验组大鼠腹腔注射氯化锂以及匹鲁卡品,对照组注射生理盐水,观察其行为学特征,并用RT-PCR方法检测大鼠海马齿状回内细胞因子IL-1 mRNA的动态表达变化。结果:大鼠腹腔注射锂-匹鲁卡品后30 min内相继出现严重癫痫持续状态发作,实验组各种细胞因子在严重癫痫持续发作后1 h表达水平开始明显增加(P<0.05),随着时间的延长表达逐渐增多,IL-1β的表达于发作后6 h达高峰,IL-1ra和IL-1R1则在发作后12 h达高峰,随后表达逐渐下降,各因子到发作后48 h表达降低但仍高于对照组表达水平(P<0.05)。结论:癫痫发作后急性期细胞因子IL-1β、IL-1ra及其受体IL-1R1在不同时间点均有增加,提示炎性因子参与了癫痫发作以及癫痫发作后脑损伤。
Objective: To observe the changes of mRNA expression of IL-1 (IL-1R, IL-1β, IL-1ra) in hippocampus dentate gyrus after seizure in rats and to explore the role of IL-1 in seizure. Methods: The rats were randomly divided into control group and experimental group. Rats in the experimental group were injected intraperitoneally with lithium chloride and pilocarpine. The control group was injected with normal saline, and the behavioral characteristics were observed. The dynamic expression of IL-1 mRNA in hippocampal dentate gyrus was detected by RT-PCR . Results: Severe epileptic seizures occurred successively within 30 min after intraperitoneal injection of lithium-pilocarpine in rats. The expression levels of various cytokines increased significantly at 1 h after severe epileptic seizure (P <0.05) IL-1β and IL-1R1 reached their peak at 12 h after onset, then decreased gradually, and each factor was expressed at 48 h after onset Decreased but still higher than the control group (P <0.05). Conclusion: The levels of IL-1β, IL-1ra and IL-1R1 in the acute phase after epileptic seizures increased at different time points, suggesting that inflammatory cytokines are involved in seizures and brain injury after seizure.