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目的 :建立雌激素缺乏的动物模型并进行实验性正畸牙齿移动。方法 :本实验选用3m龄健康雌性♀SD大鼠 5 6只 ,随机分成实验组 (切除卵巢 )和对照组 (假手术 ) ,分别于术前及术后的不同时间点采血 ,检测血清雌二醇浓度及血清碱性磷酸酶活性 ,并分别于卵巢切除后的第 1、 2、 4、 6w进行实验性牙齿移动 ,牵引大鼠第一磨牙向近中移动。结果 :雌二醇于手术后的d1即开始下降 ,术后d9降低到很低水平 (两组比较P <0 0 0 1)。血清碱性磷酸酶于术后第一周开始上升 ,术后d42达 32 7IU ,OVX组是对照组的 5倍。两组动物牙齿移动距离表明 ,雌激素缺乏组正畸牙齿移动的速度快 (P <0 0 1)。结论 :卵巢切除是建立雌激素缺乏模型的好方法 ,雌激素作为影响骨代谢的重要因素 ,它可作用于破骨细胞和成骨细胞 ,改变二者的功能状态与活性 ,这可能是造成两组动物正畸牙齿移动以及血清ALP含量差异的主要原因
Objective: To establish an animal model of estrogen deficiency and to conduct experimental orthodontic tooth movement. Methods: In this study, 56 healthy female SD rats of 3m age were randomly divided into experimental group (ovariectomized) and control group (sham operation). Blood samples were taken at different time points before and after surgery. Serum estradiol Alcohol concentration and serum alkaline phosphatase activity. The experimental tooth movement was performed on the 1st, 2nd, 4th and 6th weeks after ovariectomy, respectively. Results: Estradiol began to decrease at day d after operation and d9 decreased to a very low level after operation (P <0 01). Serum alkaline phosphatase began to rise in the first week after operation, reaching 32 7 IU after operation and 5-fold higher in the OVX group than in the control group. Tooth movement between the two groups showed that orthodontic tooth movement was faster in the estrogen-deficient group (P <0.01). Conclusion: Ovariectomy is a good way to establish a model of estrogen deficiency. As an important factor that affects bone metabolism, estrogen can act on osteoclasts and osteoblasts to change their functional status and activity, which may result in two Group orthodontic tooth movement and serum ALP content of the main reasons