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目的：检测肝癌组织中唾液酸酶的活性，探讨肝癌组织中神经节苷脂谱改变（ＧＤ３含量增加）的机理。方法：以蓖麻凝集素Ⅱ与乳糖基的特异结合为基础，用神经节苷脂ＧＭ３作为水解底物包被于９６孔板，在唾液酸酶的作用下，糖链末端的唾液酸被切除而暴露出乳糖残基，用生物素标记的蓖麻凝集素与后者结合，再用ＡＢＣ法测定结合量即可检测唾液酸酶活性。结果：肝癌组织中不管是可溶性的还是膜结合性的唾液酸酶，其活性都明显低于其癌旁组织。结论：肝癌组织中神经节苷脂ＧＤ３增加的原因不仅与ＧＤ３合成酶活性的增高有关，还与其水解酶唾液酸酶活性的降低相关。
OBJECTIVE: To detect the activity of sialidase in hepatocellular carcinoma and to explore the mechanism of changes in ganglioside profile (increased GD3 content) in hepatocellular carcinoma. Methods: Based on the specific binding of castor lectin II and lactosyl group, ganglioside GM3 was used as a hydrolyzing substrate to coat 96-well plates. Under the action of sialidase, the sialic acid at the end of the sugar chain was removed. The lactose residue is exposed and the biotin-labeled castor lectin is combined with the latter, and the sialidase activity can be detected by measuring the amount of binding by the ABC method. RESULTS: The activity of soluble or membrane-bound sialidase in HCC tissue was significantly lower than that in its adjacent tissues. Conclusion: The increase of ganglioside GD3 in hepatocellular carcinoma is not only related to the increase of GD3 synthetase activity, but also related to the decrease of sialidase activity of hydrolase.