论文部分内容阅读
目的建立检测幽门螺杆菌（Ｈｐ）的更为敏感的聚合酶链反应（ＰＣＲ），并探讨它在Ｈｐ感染的诊断及根除效果判定中的应用．方法以一对合成的与Ｈｐ１６ＳｒＲＮＡ基因互补的寡核苷酸为引物（ＣＰ１／ＣＰ２），建立了从胃粘膜中检测Ｈｐ的ＰＣＲ反应，并与常规检测方法进行比较．结果ＰＣＲ方法检测Ｈｐ标准菌株及５０株临床分离菌株均产生５００ｂｐ片段，检出的最小ＤＮＡ量为０１ｐｇ，相当于１００个细菌细胞；所有１３株其它细菌及无Ｈｐ感染的人胃粘膜则无扩增产物出现．用该方法检测９６名初诊患者Ｈｐ感染情况及２１名药物治疗后患者Ｈｐ根除情况，并与胃粘膜活组织尿素酶试验、细菌培养及银染色方法比较，证实ＰＣＲ方法可以检出常规方法不能检出的少量Ｈｐ．结论ＰＣＲ是检测Ｈｐ的最为敏感的方法，有助于Ｈｐ感染的诊断和Ｈｐ根除的精确判断
Objective To establish a more sensitive polymerase chain reaction (PCR) for the detection of Helicobacter pylori (Hp) and to explore its application in diagnosis of Hp infection and determination of eradication effect. Methods The PCR reaction of detecting Hp from gastric mucosa was established by using a pair of synthetic oligonucleotides complementary to Hp16SrRNA as primers (CP1 / CP2) and compared with routine detection methods. Results 500 bp fragments of Hp standard strains and 50 clinical isolates were detected by PCR. The minimum detectable DNA amount was 01 pg, equivalent to 100 bacterial cells. All 13 strains of other bacteria and human gastric mucosa without Hp infection No amplification products appear. This method was used to detect Hp infection in 96 newly diagnosed patients and Hp eradication in 21 patients after drug treatment. Comparing with gastric mucosa urease test, bacterial culture and silver staining, it was confirmed that PCR method could detect routine Hp infection A small amount of Hp out. Conclusion PCR is the most sensitive method for detecting Hp, which is helpful for the diagnosis of Hp infection and the accurate judgment of Hp eradication