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目的:对鸡眼草高抗氧化活性萃取部进行筛选,测定其黄酮和总酚含量。方法:采用超氧阴离子()、羟自由基(·OH)和DPPH·清除实验以及还原能力实验评价鸡眼草不同极性萃取部的抗氧化能力,分别用NaNO2-Al(NO3)3-NaOH比色法和Folin-Ciocalteu试剂法测其黄酮与多酚含量。结果:鸡眼草各萃取部清除·OH能力的强弱顺序为乙酸乙酯部位>正丁醇部位>VC>氯仿部位>石油醚部位;对的清除结果为乙酸乙酯部位和正丁醇部位的清除效果与VC相当,氯仿部位和石油醚部位的清除效果差于VC;各萃取部位对DPPH·自由基的清除能力均弱于VC,石油醚部位和氯仿部位的IC50均显著高于乙酸乙酯部位和正丁醇部位(P<0.05);还原能力的大小顺序为VC>乙酸乙酯部位>正丁醇部位>氯仿部位>石油醚部位。乙酸乙酯部位的黄酮和总酚含量均显著高于正丁醇部位(P<0.05)。结论:鸡眼草抗氧化性最强的萃取部位是乙酸乙酯部位,其次为正丁醇部位,石油醚部位最差,黄酮和多酚类可能是其抗氧化活性成分。
OBJECTIVE: To screen the extract of Cornus officinalis with high antioxidant activity and determine its flavonoids and total phenols content. Methods: The anti-oxidative ability of extracts from different polarity fractions of A. japonicus was evaluated by the experiments of superoxide anion (· OH), DPPH · scavenging activity and reducing ability. The effects of NaNO2-Al (NO3) 3-NaOH ratio Colorimetry and Folin-Ciocalteu reagent test flavonoids and polyphenols content. Results: The order of the ability of scavenging OH on the extracts was: ethyl acetate fraction> n-butanol fraction> VC> chloroform fraction> petroleum ether fraction. The scavenging results were the removal of ethyl acetate fraction and n-butanol fraction The effect was similar to that of VC, the removal efficiency of chloroform fraction and petroleum ether fraction was lower than that of VC. The scavenging capacity of DPPH · free radical was weaker than that of VC, and the IC50 of petroleum ether fraction and chloroform fraction was significantly higher than that of ethyl acetate fraction And n-butanol (P <0.05). The order of reducing ability was VC> ethyl acetate> n-butanol> chloroform> petroleum ether. The contents of flavonoids and total phenols in ethyl acetate fraction were significantly higher than those in n-butanol fraction (P <0.05). CONCLUSION: The strongest antioxidative activity of Cornus officinalis is the ethyl acetate fraction, followed by n-butanol fraction and the petroleum ether fraction is the worst. Flavonoids and polyphenols may be their antioxidative components.