论文部分内容阅读
目的:观察复健片对脑梗死大鼠脑组织RhoA、ROCK2、MLCP蛋白的影响,探讨其解除神经再生抑制的机制。方法:30只SD大鼠随机分为正常对照组(5只)、假手术组(5只)、模型组(10只)和药物组(10只)。用改良的Longa E Z法制备大脑中动脉阻塞大鼠模型,药物组灌胃给予复健片水溶液,余各组分别灌胃给予同剂量的蒸馏水。用Western Blot观察模型大鼠梗死周边区脑RhoA、ROCK2、MLCP蛋白的表达。结果:药物组大鼠脑组织中RhoA、ROCK2、MLCP表达虽然较正常对照组增多,但较模型组明显减少,二者之间有显著性差异(P<0.01)。结论:复健片可抑制Rho/ROCK通路信号因子在脑梗死后的表达,从而促进神经发生。
Objective: To observe the effect of rehabilitation tablet on RhoA, ROCK2 and MLCP protein in cerebral infarction rats and to explore its mechanism of relieving the inhibition of nerve regeneration. Methods: Thirty SD rats were randomly divided into normal control group (n = 5), sham operation group (n = 5), model group (n = 10) and drug group (n = 10). A rat model of middle cerebral artery occlusion was established by modified Longa E Z method. The drug group was given ganoderma lucidum by gavage, and the rest groups were given the same dose of distilled water by gavage respectively. Western Blot was used to observe the expression of RhoA, ROCK2 and MLCP in the peri-infarct zone of the model rats. Results: Compared with the model group, the expression of RhoA, ROCK2 and MLCP in the brain tissue of the drug-treated rats increased significantly compared with that of the normal control group, and the difference was significant (P <0.01). Conclusion: FuJian tablets can inhibit the expression of Rho / ROCK pathway signaling molecules after cerebral infarction and thus promote neurogenesis.