目的观察NADPH氧化酶的亚基p47phox对血管平滑肌细胞胆固醇代谢的影响。方法p47phox基因敲除小鼠的主动脉血管平滑肌细胞与10mg/L水溶性胆固醇共孵育72h,然后用0.3mg/L凝血酶处理10min。采用免疫组织化学法、油红O染色、real-time PCR、Western blot及细胞内胆固醇测定法,观察 Objective To observe the effect of NADPH oxidase subunit p47phox on cholesterol metabolism in vascular smooth muscle cells. Methods Aortic vascular smooth muscle cells from p47phox knockout mice were co-incubated with 10 mg / L water-soluble cholesterol for 72 h and then treated with 0.3 mg / L thrombin for 10 min. Immunohistochemistry, oil red O staining, real-time PCR, Western blot and intracellular cholesterol assay were used to observe