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目的:研究Sorcin蛋白在胃癌及癌周组织中的表达及其在胃癌发生发展中的可能作用机制。方法:应用免疫组化检测Sorcin在85例胃癌及癌周组织中的表达情况;转染Sorcin基因至正常胃粘膜上皮细胞GES-1,Western blot验证转染组及对照组Sorcin表达情况;MTT法检测细胞存活率。结果:Sorcin在胃癌组织中的表达明显高于癌旁组织(p<0.01);与对照组相比,Sorcin高表达明显增强细胞抗凋亡能力。结论:Sorcin蛋白质在胃癌中高表达,其可能通过调控细胞凋亡途径参与胃癌恶性生物学行为,有望成为潜在的诊断胃癌、判断预后的分子标志物。
Objective: To investigate the expression of Sorcin protein in gastric cancer and pericarcinoma and its possible mechanism in the development of gastric cancer. Methods: Sorcin was detected by immunohistochemistry in 85 cases of gastric cancer and pericarcinoma tissues. Sorcin gene was transfected into normal gastric mucosa epithelial cells GES-1. The expression of Sorcin in transfected and control groups was verified by Western blot. MTT assay Cell viability was measured. Results: The expression of Sorcin in gastric cancer tissues was significantly higher than that in adjacent tissues (p <0.01). Compared with the control group, Sorcin overexpression significantly increased the anti-apoptotic ability of cells. Conclusion: Sorcin protein is highly expressed in gastric cancer. It may be involved in the malignant biological behavior of gastric cancer by regulating apoptosis pathway. It may become a potential molecular marker for diagnosis of gastric cancer and prognosis.