目的：探讨人唾液对正常及肿瘤细胞在体外粘附有无不同影响。方法：接种ＮＩＨ—３Ｔ３及ＭＥＣ－１细胞在预先涂有或未涂唾液的２４孔培养板上，用细胞计数法分别在接种后２、４、６和８ｈ计数贴壁细胞。结果：ＮＩＨ－３Ｔ３细胞及ＭＥＣ－１细胞在涂有唾液的塑料底物上在２、４、６、和８ｈ其贴壁抑制率分别为３５％、４１％、２６％、３４％和１０％、９％、８％、４％。结论：人全唾液能抑制体外培养的ＮＩＨ－３Ｔ３细胞的粘附，但对ＭＥＣ－１细胞的影响不大。 Objective: To investigate whether human saliva has different effects on the adhesion of normal and tumor cells in vitro. METHODS: NIH-3T3 and MEC-1 cells were seeded on 24-well plates with or without saliva in advance and the adherent cells were counted at 2, 4, 6 and 8 h after inoculation, respectively, using the cell counting method. Results: The adhesion inhibition rates of NIH-3T3 cells and MEC-1 cells on plastic substrates coated with saliva were 35%, 41%, 26%, 34% and 10% at 2, 4, 6, , 9%, 8%, 4%. Conclusion: Human whole saliva can inhibit the adhesion of NIH-3T3 cells cultured in vitro, but it has little effect on MEC-1 cells.