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目的：为调查海南省老年性痴呆患者载脂蛋白Ｅ（Ａｐｏ Ｅ）基因频率，建立一种简便、快速、准确的人载脂蛋白Ｅ基因型检测方法。方法：应用碘化钠法提取模板ＤＮＡ，聚合酶链反应（ＰＣＲ）特异性扩增Ａｐｏ Ｅ基因含１１２ 位和１５８ 位氨基酸的基因序列，扩增产物用限制内切酶Ｈｈａ Ｉ酶切，聚丙烯酰胺凝胶电泳后，观察酶切位点的限制性片段长度多态性（ＲＦＩＰ）图谱。结果：碘化钠法提取模板ＤＮＡ 与经典法无差异；运用ＰＣＲ－ ＲＦＩＰ法检测３０ 名健康人Ａｐｏ Ｅ基因型，实验成功。结论：该方法简单、快速、准确，适合于一般实验室开展Ａｐｏ Ｅ基因型调查
Objective: To establish a simple, rapid and accurate method for detecting human apolipoprotein E genotypes in order to investigate the frequency of Apo E gene in patients with senile dementia in Hainan Province. Methods: The template DNA was extracted by sodium iodide method. The gene sequences of 112 and 158 amino acids of Apo E gene were amplified by polymerase chain reaction (PCR). The amplified product was digested with restriction endonuclease Hha I and poly After acrylamide gel electrophoresis, restriction fragment length polymorphism (RFIP) patterns were observed. Results: There was no difference between the template DNA extracted by sodium iodide and the classical method. PCR-RFIP was used to detect the Apo E genotype of 30 healthy people. The experiment was successful. Conclusion: The method is simple, rapid and accurate and suitable for general laboratory investigation of Apo E genotype