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目的 :研究中药复方 96 2胶囊改善基底前脑损毁大鼠学习记忆功能下降的作用及对中枢兴奋性的影响。方法 :采用鹅膏覃氨酸基底前脑注射造成拟阿尔茨海默病大鼠模型 ,实验分为假手术、模型、小剂量 (0 .45g/kg)、中剂量 (0 .9g/kg)和大剂量 (1.8g/kg) 5组 ,采用灌胃给药途径给药 1个月 ,分别用水迷宫、避暗反应及旷场分析对动物学习记忆功能及兴奋性进行评价。结果 :中药复方小、中剂量组均能显著缩短水迷宫游出时间 (P <0 .0 1) ,大剂量有趋势 ;3个剂量组均减少错误次数 (P <0 .0 1)。避暗反应表明 ,96 2各剂量组的成绩均优于模型组 ,其中 96 2大剂量平均错误积分及潜伏期有显著性差异 (P <0 .0 5 )。旷场分析发现该药对模型大鼠兴奋性无显著影响。结论 :中药复方 96 2具有改善基底前脑损毁大鼠学习记忆功能 ,对兴奋性无显著影响。
Objective : To study the effect of Chinese compound prescription 96 2 capsules on improving the learning and memory function of rats with basal frontal brain damage and its effect on central excitability. METHODS: The rat model of Alzheimer’s disease was induced by ibotenate basal forebrain injection. The experiment was divided into sham operation, model, low dose (0. 45g/kg), medium dose (0.9g/kg). Five groups of large dose (1.8g/kg) were administered by intragastric administration for one month. The learning and memory function and excitability of the animals were evaluated by water maze, avoid dark reaction and open field analysis. RESULTS: The small and middle doses of Chinese herbal compound group could significantly shorten the water maze escape time (P < 0.01), and there was a tendency for large doses; the three dose groups all reduced the number of errors (P <0.01). The dark avoidance response showed that the performance of the 96 2 dose groups was better than that of the model group, and the 96 2 large dose mean error scores and latency were significantly different (P < 0.05). The open-field analysis found that the drug had no significant effect on the excitability of the model rats. Conclusion : Compound 96 2 of Chinese herbal medicine can improve the learning and memory function of rats with basal frontal brain damage and has no significant effect on excitability.