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目的 观察B7 1和IL 12基因表达对人肝癌细胞免疫原性的影响。方法 分别将B7 1和IL 12基因以逆转录病毒介导转染HepG2细胞。阳性克隆细胞与健康人外周血淋巴细胞 (PBL)混合培养后 ,用流式细胞仪检测PBL表面Ⅰ类人白细胞抗原 (HLA Ⅰ )分子表达 ,以MTT法检测PBL的特异性杀伤活性及杀伤K5 6 2细胞的活性。结果 混合培养后HepG2 /B7 1和HepG2 /IL 12细胞组PBL表面的HLA Ⅰ分子表达分别较HepG2 /neo细胞组增加16 .95 %和 14 .71% (P <0 .0 5 ) ;与HepG2 /neo组比较 ,HepG2 /B7 1组PBL特异性杀伤活性增高 12 .5 % (P <0 .0 5 ) ,HepG2 /IL 12组PBL的特异性杀伤活性无明显增高 (P >0 .0 5 )。HepG2 /B7 1、HepG2 /IL 12细胞活化的PBL对K5 6 2细胞的杀伤活性较HepG2 /neo组分别增高 19.38%和 14 .78% (P <0 .0 5 )。 结论 B7 1和IL 12分子均能增强免疫细胞对肝癌细胞的识别和杀伤 ,从而有效增强肝癌细胞的免疫原性
Objective To observe the effect of B7 1 and IL 12 gene expression on the immunogenicity of human hepatocellular carcinoma cells. Methods The B7 1 and IL 12 genes were transfected into HepG2 cells by retrovirus. After positive clone cells were mixed with healthy human peripheral blood lymphocytes (PBL), the expression of type I human leukocyte antigen (HLA I) on the surface of PBL was detected by flow cytometry, and the specific killing activity of PBL and K5 were measured by MTT assay. 6 2 cell activity. Results After mixed culture, the expression of HLA I molecules on the surface of HepG2/B7 1 and HepG2/IL 12 cells group was increased by 16.95% and 14.71% (P < 0.05) compared with that of HepG2/neo cells, respectively; and HepG2 Compared with the neo group, the specific killing activity of PBL in the HepG2/B7 1 group increased by 12.5% (P < 0.05), and the specific killing activity of the PBL in the HepG2/IL 12 group did not increase significantly (P > 0.05). ). The killing activity of HepG2/B7 1 and HepG2/IL 12 activated PBL on K562 cells was 19.38% and 14.78% higher than HepG2/neo group, respectively (P < 0.05). Conclusion Both B7 1 and IL 12 molecules can enhance the recognition and killing of hepatocellular carcinoma cells by immune cells, thus effectively enhancing the immunogenicity of hepatoma cells.