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采用荧光定量 PCR 方法观察45例慢性丙型肝炎(简称慢丙肝)治疗前后血清 HCV RNA 含量变化,探讨治疗前血清 HCVRNA 水平等因素对干扰素治疗应答的影响。材料与方法一、对象 45例慢丙肝为我院1993年元月至1995年12月住院患者,其中男性33例,女性12例,平均年龄42.5±9.3岁(21～65岁)。慢丙肝诊断依据为:病程>6个月,ALT 治疗前为正常上限的2倍或2倍以上,抗 HCV 及 HCV RNA 均阳性,其中经肝活检征实者21例,所有病例 HBsAg 均阴性。二、方法 HCV PNA 定量检测:按荧光定量 PCR 检测系统操作说明书进行。标化 HCV RNA 及定量 PCR 扩增引物由美国 Biotronics 公司提供。三、治疗方法及疗效评价将观察病例随机分为干扰素(IFN)300万单位(MU)组22例和 IFN450MU 组23例,每周3次
Fluorescent quantitative PCR method was used to observe the changes of serum HCV RNA levels in 45 patients with chronic hepatitis C before and after treatment, and to explore the influence of pretreatment serum HCV RNA levels on the response to interferon treatment. Materials and Methods First, the object 45 cases of chronic hepatitis C hospital from January 1993 to December 1995 hospitalized patients, including 33 males and 12 females, mean age 42.5 ± 9.3 years (21 to 65 years). The diagnosis of chronic hepatitis C was based on the following: duration of disease> 6 months, 2 or more times the upper limit of normal before ALT treatment, anti-HCV and HCV RNA were positive, of which 21 were confirmed by liver biopsy, all cases were negative HBsAg. Second, the method of quantitative detection of HCV PNA: According to fluorescence quantitative PCR detection system operating instructions. Standardized HCV RNA and quantitative PCR amplification primers provided by the United States Biotronics. Third, the treatment and efficacy evaluation The observation cases were randomly divided into interferon (IFN) 3000000 unit (MU) group 22 cases and IFN 450MU group 23 cases, 3 times a week