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目的研究生长抑素ｓｔｉｌａｍｉｎ（ＳＳ－１４肽）和ｓａｎｄｏｓｔａｔｉｎ（ＳＳ－８肽）对人晶上皮细胞（ＨＬＥＣ）增殖及胶原合成的影响。方法采用ＭＴＴ比色法和３Ｈ－脯氨酸（３Ｈ－Ｐｒｏｌｉｎｅ）掺入法检测了ＳＳ－１４肽和ＳＳ－８肽对人晶体上皮细胞（ＨＬＥＣ）增殖及胶原合成的抑制作用。结果ＳＳ－８肽浓度为１０－１０ｍｏｌ／Ｌ、ＳＳ－１４肽浓度为１０－９ｍｏｌ／Ｌ时能明显抑制ＨＬＥＣ增殖和胶原合成，且呈剂量依赖关系，５０％抑制浓度（ＩＤ５０）ＳＳ－８肽为１０－８ｍｏｌ／Ｌ，ＳＳ－１４肽为１０－７ｍｏｌ／Ｌ。结论ＳＳ－１４肽和ＳＳ－８肽可以通过抑制ＨＬＥＣ的增殖及胶原的合成而抑制后囊混浊。
Objective To investigate the effects of stilamin (SS-14 peptide) and sandostatin (SS-8 peptide) on the proliferation and collagen synthesis of human epithelial cells (HLECs). Methods The inhibitory effects of SS-14 peptide and SS-8 peptide on the proliferation and collagen synthesis of human lens epithelial cells (HLECs) were examined by MTT assay and 3H-Proline incorporation assay. Results SS-8 peptide concentration of 10-10mol / L, SS-14 peptide concentration of 10-9mol / L significantly inhibited HLEC proliferation and collagen synthesis in a dose-dependent manner, 50% inhibitory concentration The peptide is 10-8 mol / L, and the SS-14 peptide is 10-7 mol / L. Conclusions SS-14 peptide and SS-8 peptide can inhibit posterior capsule opacity by inhibiting the proliferation of HLEC and collagen synthesis.