牛黄解毒片中胆酸的鉴别,中国药典1985年版一部第388页规定用薄层层析法。但在实际工作中发现,按药典规定方法做薄层层析鉴别时,很难观察到对照溶液的斑点。其原因我们认为这是由于配制对照溶液时,胆酸在氯仿中很难溶解,即使是水浴加热并搅拌研磨也不能使其完全溶解,使对照溶液的胆酸实际浓度较低所致,因此,对该法做了改进,在配 The identification of cholic acid in Niuhuang Jiedu Tablets is stipulated in the Chinese Pharmacopoeia, 1985 edition, No. 388, using thin-layer chromatography. However, in practical work, it has been found that it is difficult to observe the spot of the control solution when the thin layer chromatography is used for the identification according to the method prescribed by the Pharmacopoeia. The reason for this is that we believe that because of the difficulty in dissolving cholic acid in chloroform when preparing the control solution, even if it is heated and stirred in a water bath, it cannot be completely dissolved, resulting in a lower concentration of bile acids in the control solution. Therefore, The law has been improved to match