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1 概述 1.1背景:脂肪酸乙酯(FAEEs)乃非氧化乙醇的代谢产物,因其存在于人的头发中,故可作为过量摄入乙醇的长期标志物. 1.2方法:从中剪取发样:19个正行治疗的嗜酒者、10个因过量摄入乙醇致死者、13个适量饮酒者和5 个滴酒不沾者.对发样的1～12诸节段中所含4种FAEE(十四烷酸乙酯、十六烷酸乙酯、油酸乙酯和硬脂酸乙酯)予以分析.所用方法是:先用正庚烷对发样脱脂,尔后用二甲亚砜与正庚烷的混合物萃取,再对萃取物进行HS-SPME和CC-MS,用从头发表面分析FAEEs同样的方法分析正庚烷的洗液. 1.3 结果:4种FAEE在近发根的0～6cm头发节段中的浓度和分别是:过量摄入乙醇致死者,2.5～13.5
1 Overview 1.1 Background: Fatty Ethyl Esters (FAEEs) are non-oxidized metabolites of ethanol and, because of their presence in human hair, can serve as long-term markers of excessive ethanol intake.1.2 Methods: Hair samples were cut from: 19 10 alcoholic deaths due to overdose, 13 moderate drinkers, and 5 non-alcoholic individuals.Five FAEEs (10 Tetradecanoic acid ethyl ester, hexadecanoic acid ethyl ester, oleic acid ethyl ester and stearic acid ethyl ester) were analyzed by the method used as follows: firstly, the hair sample was degreased with n-heptane, then dimethylsulfoxide and n-heptane Alkane mixture and the extract was subjected to HS-SPME and CC-MS and the n-heptane washings were analyzed in the same way as FAEEs on the hair surface.1.3 Results: Four FAEEs were found in 0-6 cm of the proximal hair root The concentrations in the segments were respectively: excess ethanol intake to the deceased, 2.5 to 13.5