论文部分内容阅读
目的:探索胃粘膜良、恶性病变中,细胞内rasP~(21)蛋白含量的变化规律与胃癌发生发展的关系.方法:应用图像分析系统对97例胃正常粘膜、各级异型增生和癌进行了抗ras基因产物P~(21)抗体免疫组化标记物的定量测定.结果:P~(21)阳性细胞胞浆平均吸光度值(A)在正常胃粘膜、轻、中、重度异型增生和高分化腺癌中分别为57.082、57.896、62.531、65.750、66.139,其中A在重度异型增生明显高于正常胃粘膜与轻度异型增生(P<0 .05～0.01),而与高分化腺癌接近(P>0.05).结论:提示P~(21)过表达与胃癌发生与分化程度可能有密切关系.图像分析定量测定能客观地反映胃粘膜不同病变中P~(21)蛋白的相对含量,对早期癌变细胞的检出有一定意义.
Objective: To explore the relationship between the changes of intracellular rasP 21 protein and the occurrence and development of gastric cancer in benign and malignant gastric mucosal lesions. Methods: Apply image analysis system to 97 cases of normal gastric mucosa, dysplasia and cancer at different levels. Quantitative determination of anti-ras gene product P~(21) antibody immunohistochemical markers. Results: P~(21) positive cells cytoplasmic average absorbance (A) in normal gastric mucosa, mild, moderate, severe dysplasia and In well-differentiated adenocarcinomas, they were 57.082, 57.896, 62.531, 65.750, and 66.139, respectively, in which A was significantly higher in severe dysplasia than in normal gastric mucosa and mild dysplasia (P < 0.05), whereas in well-differentiated adenocarcinomas. Close (P>0.05). Conclusion: It is suggested that P21 expression may be closely related to the occurrence and differentiation of gastric cancer. Quantitative image analysis can objectively reflect the relative content of P21 protein in different gastric lesions. The detection of early cancerous cells has a certain significance.