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目的 探讨胃癌及其癌前病变中 ras,c- erb B- 2 ,p5 3癌基因蛋白产物的表达分布特点及其在胃癌发生发展中的作用 .方法 应用免疫组化 ABC方法 ,对 5 8例胃癌、31例胃粘膜上皮异型增生和 11例正常胃粘膜组织中 ,鼠抗人 m Ab ras基因 p2 1蛋白、兔抗人 c- erb B- 2原癌基因产物多克隆抗体p185 ,鼠抗人 m Ab p5 3蛋白进行了检测 .结果 p2 1在正常胃粘膜和轻、中、重度异型增生和胃癌中的表达率分别为2 7.3% ,30 .0 % ,46 .2 % ,75 .0 %和 72 .4% ;其表达率在重度异型增生和胃癌中显著高于正常胃粘膜和轻、中度异型增生(P<0 .0 5 ) ;p185在正常胃粘膜和轻、中度异型增生中无表达 ,在重度异型增生和癌中的阳性表达率分别为 2 5 .0 % ,18.9% ,各组间无显著性差异 (P>0 .0 5 ) ;p5 3仅在癌中有表达 ,表达率 2 9.3% ,明显高于非癌组织 (P<0 .0 5 ) .胃癌中两种以上癌基因蛋白同时表达率明显高于非癌组织 (P<0 .0 5 ) .结论 ras基因可能作用于胃癌发生的早期阶段 ,c-erb B- 2 ,p5 3基因可能作用于胃癌发生的较晚期 .并认为胃癌发生需要多基因协同作用
Objective To investigate the expression and distribution of ras, c-erb B-2, p53 oncogene protein products in gastric cancer and its precancerous lesions and its role in the development of gastric cancer. Methods Immunohistochemical ABC method was used in 58 cases. Gastric cancer, 31 cases of gastric epithelial dysplasia, and 11 cases of normal gastric mucosa, mouse anti-human m Ab ras gene p21 protein, rabbit anti-human c-erb B-2 proto-oncogene product polyclonal antibody p185, mouse anti-human m Ab p53 protein was detected. Results The expression rates of p21 in normal gastric mucosa, mild, moderate, severe dysplasia and gastric cancer were 27.3%, 30.0 %, 46.2%, 75.0 % respectively. And 72.4%; its expression rate was significantly higher in severe dysplasia and gastric cancer than normal gastric mucosa and mild to moderate dysplasia (P < 0.05); p185 in normal gastric mucosa and mild to moderate dysplasia No positive expression was observed in severe dysplasia and carcinoma. The positive expression rates were 25.0% and 18.9%, respectively. There was no significant difference among the groups (P>0.05); p53 was only expressed in carcinomas. The expression rate was 2 9.3%, which was significantly higher than that of non-cancer tissues (P<0.05). The simultaneous expression rate of two or more oncogene proteins in gastric cancer was significantly higher than that in non-cancer tissues. Texture (P <0 .0 5). Conclusion The possible role of ras gene in the early stage of gastric cancer, c-erb B- 2, p5 3 gene possible role in gastric cancer later stage. Gastric and that require multiple genetic synergy