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应用间接酶标抗体免疫组化法测出了53例新鲜精液、5例陈旧精斑及40例阴道分泌液中的精子与阴道脱落上皮细胞的ABO血型,30例精子与不同血型分泌型阴道分泌液孵育,未发现精子吸附阴道液中血型物质的现象,同时发现人类睾丸曲精细管中部份生精细胞、精子细胞,精子;直细精管部份上皮细胞、精液、精子;睾丸网大部份上皮细胞及副睾管中的精液与精子均含ABH抗原,故认为精子上的ABH抗原主要是精子固有抗原,13例性交后阴道内容物中精子的ABO型测定结果:7例与供者血型吻合,6例不吻合。6例中5例从O型精子中测出了女方分泌型阴道分泌液中的A或AB物质,1例B型精子未测出B及H抗原,文中对这种现象进行了讨论。
Indirect enzyme-linked immunosorbent assay was used to detect the ABO blood type of sperm and vaginal epithelial cells in 53 cases of fresh semen, 5 cases of old semen and 40 cases of vaginal secretions, 30 cases of seminal secretions with different types of vaginal secretion Liquid incubation, no sperm absorption of blood substances in the vaginal fluid phenomenon, also found in human testicular seminiferous tubules in part of spermatogenic cells, sperm cells, sperm; spermatida epithelial cells, semen, sperm; large testis network Some epithelial cells and the epididymal seminal fluid and sperm contain ABH antigen, so that the sperm ABH antigen is mainly sperm-specific antigen, 13 cases of vaginal contents after sexual intercourse sperm ABO type determination results: 7 cases and for Blood type anastomosis, 6 cases do not match. Among 6 cases, 5 cases detected A or AB in female secretory vaginal secretions from O-type spermatozoa, and 1 case did not detect B and H antigens in type B spermatozoa. This phenomenon was discussed in this paper.