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目的研究青芥辣对离体肝吸虫囊蚴的杀灭作用。方法采用人工消化法从淡水鱼中获取囊蚴,分组(囊蚴20个/组),将其置于定量青芥辣软膏中,分别作用不同时间,显微镜下观察囊蚴的活动情况。结果青芥辣对肝吸虫囊蚴作用1～6min后,囊蚴内后尾蚴活动加强;作用7～180min后囊蚴活动力降低甚至开始死亡,死亡率范围为11.76%～100%;作用210min以后囊蚴全部死亡,死亡率达到100%。对照组囊蚴形态和后尾蚴活动无改变。结论青芥辣对肝吸虫囊蚴具有刺激和轻度杀灭作用,但在短时间内没有杀灭作用,所以期望用青芥辣杀灭肝吸虫囊蚴来达到预防肝吸虫感染的做法不可取。
Objective To study the killing effect of green mustard and spleen on isolated metacercariae larvae. Methods Echinococcus granulosus was obtained from freshwater fish by artificial digestion, and divided into groups (20 per group). They were placed in quantitative green wasabi ointment, and were respectively treated with different time. The activity of metacercariae was observed under the microscope. The results showed that after 1 ~ 6 min of the action of the green mustard and spicy larvae, the post-cercariae activity of the metacercariae increased. After 7 ~ 180 min, the viability of the metacercariae decreased or even started to die, with the mortality ranged from 11.76% to 100%. After 210 min Echinococcosis all died, the mortality rate reached 100%. The control group, metacercaria morphology and post-cercariae activity did not change. Conclusions Green mustard spicy has a stimulating and mild killing effect on metacercariae, but it has no killing effect in a short period of time. Therefore, it is not advisable to use green mustard and spicy to kill metacercariae larvae to prevent liver fluke infection. .