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本文介绍用简便方法从HBsAg阳性尸肝中提取HBcAg。用PEG沉淀、DEAE·A_(50)五管提纯法从抗-HBc阳性血清中纯化抗-HBc及市售马抗人IgM诊断血清;用过碘酸钠法标记辣根过氧化物酶,制成抗-HBc及抗-HBcIgM试剂盒,经与Abbott抗-HBc试剂盒比较结果相符,应用于临床获得良好效果。
This article describes the simple and convenient method of extracting HBcAg from HBsAg positive corpses. Anti-HBc positive serum was purified by PEG-precipitation and DEAE-A_ (50) five-tube purification, and serum was detected by horseradish peroxidase (HRM). The horseradish peroxidase Anti-HBc and anti-HBcIgM kit, Abbott anti-HBc kit with the results of the comparison, the clinical application of good results.