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目的探讨ＭＴＳ１与胃癌的关系。方法３２例胃癌和３０例正常胃粘膜标本提取细胞ＤＮＡ、总ＲＮＡ，ＭＴＳ１ｃＤＮＡ探针采用随机引物法进行３２ＰｄＡＴＰ掺入标记，ＭＴＳ１与所提ＤＮＡ、ＲＮＡ进行Ｓｏｕｔｈｅｒｎ和Ｎｏｒｔｈｅｒｎ杂交分析，检测标本中有无ＭＴＳ１的缺失和ｍＲＮＡ的不表达。结果ＭＴＳ１Ｓｏｕｔｈｅｒｎ和Ｎｏｒｔｈｅｒｎ杂交分析结果显示，３２例胃癌标本ＭＴＳ１基因缺失及ｍＲＮＡ不表达率分别为３４．４％和４０．６％，明显高于正常胃粘膜组。ＭＴＳ１基因缺失及ｍＲＮＡ不表达与胃癌浸润的深度及分化程度有关，胃癌侵及肌层组ＭＴＳ１基因缺失及ｍＲＮＡ不表达率分别为５２．６％和５７．９％，胃癌未侵及肌层组分别为７．７％和１５．４％，高、中分化胃癌组ＭＴＳ１基因缺失及ｍＲＮＡ不表达率分别为７．１％和１４．３％，而低分化及印戒细胞胃癌组分别为５５．８％和６１．１％。结论胃癌的发生、发展与ＭＴＳ１基因的缺失及ｍＲＮＡ不表达有关，检测胃癌组织中ＭＴＳ１变化对胃癌的诊断，了解胃癌的生物学特征，为病人预后的评估及治疗提供新的有价值的客观依据。
Objective To investigate the relationship between MTS1 and gastric cancer. Methods DNA and total RNA were extracted from 32 gastric cancer specimens and 30 normal gastric mucosa specimens. MTS1 cDNA probe was used for 32PdATP incorporation with random primers. MTS1 was analyzed with Southern and Northern hybridizations of DNA and RNA. There was no deletion of MTS1 and no expression of mRNA. Results The results of MTS1 Southern and Northern hybridization analysis showed that the MTS1 gene deletion and mRNA non-expression rates in 32 gastric cancer specimens were 34.4% and 40.6%, respectively, which were significantly higher than those in the normal gastric mucosa group. MTS1 gene deletion and mRNA expression were related to the depth of invasion and differentiation of gastric cancer. The rate of MTS1 gene deletion and mRNA non-expression in gastric cancer invading and muscular layers were 52.6% and 57.9%, respectively. Gastric cancer did not invade the myometrium group. At 7.7% and 15.4%, respectively, the MTS1 gene deletion and mRNA non-expression rates in the high- and moderately differentiated gastric cancer groups were 7.1% and 14.3%, respectively, whereas the low-differentiation and signet-ring cell gastric cancer groups were 55. .8% and 61.1%. Conclusion The occurrence and development of gastric cancer is related to the loss of MTS1 gene and the absence of mRNA expression. The detection of MTS1 in gastric cancer tissues is a useful tool for the diagnosis of gastric cancer, to understand the biological characteristics of gastric cancer, and to provide a new valuable objective basis for the assessment and treatment of patients’ prognosis. .