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MTS1是Kamb在1994年发现的一种新的抑癌基因。在很多类型肿瘤中经常出现MTS1基因的纯合性缺失或突变。本文利用PCR方法研究了MTS1基因第2外显子在胃癌中的纯合性缺失情况,24例标本中发现2例缺失。用PCR-SSCP并结合PCR产物直接测序技术分析了24例原发性胃癌标本中MTS1基因第2外显子突变情况,只发现1例突变,此结果提示胃癌中MTS1基因突变率较低
MTS1 is a new tumor suppressor gene discovered by Kamb in 1994. Homozygous deletions or mutations of the MTS1 gene often occur in many types of tumors. In this paper, the homozygous deletion of MTS1 gene exon 2 in gastric cancer was studied by PCR. Two of 24 samples were found to be missing. The mutation of exon 2 of MTS1 gene in 24 cases of primary gastric cancer was analyzed by PCR-SSCP combined with direct sequencing of PCR products. Only one mutation was found. This result suggests that the mutation rate of MTS1 gene in gastric cancer is low.