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目的：观察用白细胞介素 ２（ｉｎｔｅｒｌｅｕｋｉｎ－２，ＩＬ－２）基因修饰小鼠骨髓来源的树突状细胞（ｄｅｎｄｒｉｃ ｃｅｌｌｓ， ＤＣ）后，ＤＣ表达细胞因子和趋化因子的变化及ＤＣ体内免疫后，小鼠体内主要脏器中ＩＬ－２表达情况。探讨其对抗原提呈微环境的改善及其可能的机制。方法：用ＥＩＪＳＡ方法检测ＤＣ培养上清和小鼠血清及脏器中ＩＬ－２的含量，用ＲＴ－ＰＣＲ方法检测ＤＣ中ＩＬ－２，ＩＦＮ－γ，ＩＬ－１２，ＧＭ－ＣＳＦ，ＩＬ－４，ＩＬ－１０，ＴＮＦ－α，ＩＬ－１－β，ＭＩＰ－１β，ＭＣＰ－１，ＲＡＮＴＥＳ和ＩＰ１０等的表达，用ＦＡＣＳ分析ＤＣ表面ＩＬ－２受体的变化。结果：经ＩＬ－２基因修饰后，ＤＣ除了分泌高水平的ＩＬ－２外，尚能分泌一定水平的ＩＦＮ－γ和ＩＬ－１２，其表面表达的ＩＬ－２Ｒα密度增加。ＲＴ－ＰＣＲ分析表明其表达多种细胞因子和趋化因子，用ＩＬ－２基因修饰的ＤＣ免疫小鼠后，血清和脏器中能检测到高水平的ＩＬ－２。结论：ＩＬ－２基因修饰ＤＣ，能促进ＤＣ表达ＩＬ－２受体，并以自分泌形式分泌高水平的ＩＬＺ，优化其抗原提呈的微环境，提示可增强ＤＣ的抗原提呈功能。
Objective: To observe the changes of cytokines and chemokines expressed in DCs and DC in vivo after modification of mouse bone marrow-derived dendritic cells (DCs) with interleukin-2 (IL-2) gene Afterwards, IL-2 expression was observed in major organs in mice. Explore its improvement of the microenvironment of antigen presentation and its possible mechanisms. Methods: The content of IL-2 in DC culture supernatant and mouse serum and organ was detected by EIJSA method. IL-2, IFN-γ, IL-12, GM-CSF and IL-4 in DC were detected by RT-PCR. Expression of IL-2, IL-10, TNF-α, IL-1-β, MIP-1β, MCP-1, RANTES and IP10, etc. The changes of IL-2 receptor on DC surface were analyzed by FACS. RESULTS: After IL-2 gene modification, DCs secreted high levels of IL-2, but secreted certain levels of IFN-γ and IL-12, and the surface expression of IL-2Rα increased. RT-PCR analysis showed that it expresses a variety of cytokines and chemokines. After immunization of mice with IL-2 gene-modified DCs, high levels of IL-2 can be detected in serum and organs. CONCLUSION: DCs modified by IL-2 gene can promote the expression of IL-2 receptors in DCs, secrete high levels of ILZ in autocrine form, and optimize the microenvironment for antigen presentation, suggesting that DCs can enhance the antigen presentation function of DCs.