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肌浆网(SR)是控制肌肉收缩和舒张的重要环节。已知大鼠的心肌收缩和舒张特性不同于其它哺乳动物,据报道大鼠心肌SR转运钙的速度也比狗、兔、豚鼠的SR为慢,这究竟是动物种属差异还是方法学问题呢?为此,本文研究了ATP、ADP与大鼠心肌SR中钙的结合之间的关系和从新估价钙结合的测定方法。微孔过滤法是研究钙结合和转运的常用方法,以往文献报道的方法各不相同,作者发现在孵育时Ca~(2+)和ATP加入的次序会影响实验结果。若先与ATP孵育再加Ca~(2+)开始反应,则Ca~(2+)的结合偏低。大鼠心脏SR具有很高的由Mg~(2+)激活的ATP酶(1.37±0.16μMol.Pi/min/mg在25℃)的活
Sarcoplasmic reticulum (SR) is an important part of controlling muscle contraction and relaxation. It is known that the contractile and diastolic characteristics of the myocardium in rats are different from those in other mammals. SR is also reported to be slower in SR than in dogs, rabbits and guinea pigs, and is this a matter of species diversity or methodological issues in animals? To this end, this paper studies the relationship between ATP and ADP and the calcium binding in the SR of rat myocardium and the method of determining the calcium binding newly. Micropore filtration method is a common method to study calcium binding and transport. The methods reported in the past are different. The authors found that the order of Ca 2+ and ATP addition during incubation will affect the experimental results. If incubated with ATP plus Ca ~ (2+) to start the reaction, the binding of Ca ~ (2+) is low. The rat heart SR has a high viability of Mg 2+ -activated ATPase (1.37 ± 0.16 μMol.Pi / min / mg at 25 ° C.)