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目的：实验观察经典缺血处理及硫酸腺嘌呤处理大鼠心肌细胞色素氧化酶和过氧化氢酶的活性变化。方法：采用ＳＤ雄性大鼠２４只，分４组：假手术组、缺血再灌注组、经典缺血预处理组、硫酸腺嘌呤预处理组。术后速取左心室前壁肌行冰冻切片，以 ＤＡＢ法测定细胞色素氧化醇和过氧化氢酶的活性。依Ｒｉｄｉｔ法行显著性检验。结果：缺血再灌注组大鼠心肌过氧化氢酶和细胞色素氧化酶损伤性反应明显，经典缺血预处理组和硫酸腺嘌呤预处理组细胞色素氧化酶、过氧化氢酶损伤性反应较之明显减轻（Ｐ＜０．０５）。结论：经典缺血预处理和硫酸腺嘌呤预处理对缺血再灌注大鼠心肌损伤有明显保护作用。
OBJECTIVE: To observe the changes of myocardial cytochrome oxidase and catalase activity induced by classical ischemia and adenine sulphate in rats. Methods: Twenty-four SD male rats were divided into 4 groups: sham operation group, ischemia-reperfusion group, classical ischemic preconditioning group and adenine sulfate pretreatment group. Postoperatively, the anterior wall of left ventricle was frozen and sliced to determine the activity of cytochrome oxidase and catalase by DAB method. According to Ridit method of significance test. Results: Myocardial catalase and cytochrome oxidase had a significant anti-ischemic reperfusion injury in ischemic-reperfusion rats. Compared with ischemic preconditioning and adenine sulphate preconditioning, cytochrome oxidase (P <0.05). Conclusion: Classical ischemic preconditioning and adenine sulphate preconditioning have significant protective effects on myocardial injury induced by ischemia / reperfusion in rats.