论文部分内容阅读
目的:分析恶性肿瘤危重病人预警蛋白(LGT-Lost Goodwill Target)指纹由阴性向阳性转变后是否出现有意义的差异指纹。方法:应用CM10弱阳离子芯片结合表面增强飞行时间质谱(Surface Enhanced Laser Desorption/Ionization Time of Flight Mass Spectrometry,SELDI-TOF-MS)技术检测21例恶性肿瘤患者(分析组)LGT指纹由阴性向阳性变异前后的血清蛋白质指纹谱,以质/核比(M/Z):11100+H~11900+H,出现丰度>10%峰簇(cluster),视为LGT阳性,反之为阴性。另选择12例肿瘤患者各1份血清样本,间隔一周检测一次,再选择5例肿瘤患者取连续两天血清标本各1份进行检测,以此作为影响因素对照组。去除与对照组相同差异指纹后,比较LGT阳性向阴性转变后差异蛋白质组指纹有无显著性。结果:分析组中恶性肿瘤患者LGT指纹由阴性向阳性变异时,有18个蛋白质指纹差异有统计学意义(P<0.05),对照组中有差异指纹44个,其中M/Z为1005、1008的指纹与分析组相同,将之剔除后,剩余了16个有统计学意义的差异蛋白质指纹,其中上调指纹的m/z(质/核比)值分别为6626、2889、6429、8866、3452、1259、3052、3699、8531、757、1146和8765。下调指纹的m/z值分别为17650、7979、778和1006。结论:SEL-DI-TOF-MS技术检测恶性肿瘤患者血清捕获的m/z:6626、2889、6429、8866、3452、1259、3052、3699、8531、757、1146和8765蛋白质指纹呈上调趋势,m/z:17650、7979、778和1006蛋白质指纹呈下调趋势,可视为恶性肿瘤LGT指纹阴转阳的差异蛋白质。
Objective: To analyze whether fingerprints of LGT-Lost Goodwill Target have significant difference after negative to positive staining. METHODS: The positive and negative LGT fingerprints of 21 malignant tumor patients (analysis group) were detected by CM10 weak cation chip combined with Surface Enhanced Laser Desorption / Ionization Time of Flight Mass Spectrometry (SELDI-TOF-MS) Before and after the serum protein fingerprinting, mass / nuclear ratio (M / Z): 11100 + H ~ 11900 + H, abundance> 10% peak cluster (cluster), as LGT positive, otherwise negative. Another 12 cases of cancer patients were selected 1 serum samples, once a week interval test, and then select 5 cases of cancer patients take two consecutive serum samples were detected as one of the influencing factors in the control group. After removing the same differential fingerprints as the control group, whether there was significant difference in the proteome fingerprints after LGT positive to negative conversion was compared. Results: There were 18 protein fingerprints in LGT fingerprints of patients with malignant tumor from malignant tumor patients to malignant tumor patients (P <0.05). There were 44 fingerprints in the control group with M / Z 1005,1008 Of the fingerprints were the same as those of the analysis group. After removing the fingerprints, 16 statistically significant differences in protein fingerprints remained, in which the m / z (mass / nucleus ratio) values of fingerprints were 6626, 2889, 6429, 8866, , 1259, 3052, 3699, 8531, 757, 1146 and 8765. Down-adjusted fingerprints m / z values were 17650,7979,778 and 1006 respectively. CONCLUSIONS: SELF-DI-TOF-MS was used to detect the m / z of sera captured by malignant tumor patients: the protein fingerprints of 6626,2889,6429,8866,3452,1259,3052,3699,8531,757,1146 and 8765 were up-regulated, The protein fingerprints of 17650, 7979, 778 and 1006 showed a downward trend, which could be regarded as the differential protein of LGT fingerprints in malignant tumors.