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对抗性番茄材料‘Hawaii 7996’分别接种强致病力青枯菌(RsH)和中等致病力青枯菌(RsM)后,利用双向电泳技术找到了两个差异表达蛋白质——谷胱甘肽S转移酶L3和Remorin 1蛋白。在利用携带八氢番茄红素脱氢酶基因PDS的TRV重组病毒载体通过叶片浸润法证明VIGS体系可行后,进一步利用该技术沉默上述两个蛋白质的对应基因GST-L3和Rem-1,并用半定量RT-PCR检测沉默效果。结果表明,分别沉默GST-L3和Rem-1的番茄植株抗性减弱,在接种中等致病力青枯菌(RsM)后青枯病发病率和病情指数都明显高于对照植株,说明基因GST-L3和Rem-1与番茄对青枯病的抗性相关。
After confrontational tomato material ’Hawaii 7996’ was inoculated with R. solanacearum (RsH) and intermediate pathogenic R. solanacearum (RsM) respectively, two differentially expressed proteins, glutathione S transferase L3 and Remorin 1 protein. After the VIGS system was proved by leaf-infiltration method using TRV recombinant virus carrying phytoene dehydrogenase gene PDS, the corresponding genes GST-L3 and Rem-1 of the above two proteins were further silenced by using the technique and half Quantitative RT-PCR detection of silencing effect. The results showed that the resistance of tomato plants with GST-L3 and Rem-1 silencing decreased respectively. The incidence and disease index of bacterial wilt after inoculation with R. solanacearum (RsM) were significantly higher than that of the control plants, indicating that GST -L3 and Rem-1 are related to tomato resistance to bacterial wilt.