传统的微量病原检测主要有直接检测法和培养基富集后检测法,前者由于病原含量低、样品杂质存留多等问题,检测灵敏度较低;后者流程繁琐、耗时费力,不能用于快速检测。而在常规PCR和ELISA基础上衍生出的新技术已广泛应用于各个领域,但这些技术几乎都集中在病原的检测部分而忽略了 The traditional detection of trace pathogens are mainly direct detection method and medium enrichment detection method, the former due to low levels of pathogens, sample impurities and other problems, the detection sensitivity is low; the latter cumbersome process, time-consuming and can not be used for fast Detection. New technologies derived from conventional PCR and ELISA have been widely used in various fields, but almost all of these techniques are focused on the pathogen detection part and ignore