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目的 研究低剂量1 4 7Pm内污染对红细胞和淋巴细胞遗传突变的影响。方法 应用骨髓和血液嗜多染与正常染红细胞微核检测以及骨髓淋巴细胞染色体畸变分析观察。结果 与正常对照组比较 ,18 5kBq g体重1 4 7Pm内污染可引起红细胞微核细胞率和淋巴细胞染色体畸变率明显增多 (P <0 0 5或 0 0 1)。然而 ,预先应用 0 37,3 7和 37Bq g体重的1 4 7Pm内污染动物 ,3d后注入18 5kBq g体重的1 4 7Pm ,红细胞微核细胞率以及淋巴细胞染色体畸变率则显著低于单纯高剂量1 4 7Pm组 (P <0 .0 5或 0 0 1) ;并且某些细胞突变与对照组比较已无显著性差异 (P >0 .0 5 )。结论 低剂量1 4 7Pm内污染预处理 ,可在较长时间内诱导红细胞和淋巴细胞的辐射适应性反应 :1 4 7Pm内污染诱导的细胞适应性反应具有较宽的剂量范围 ;与淋巴细胞染色体畸变比较 ,嗜多染与正常染红细胞微核亦是较灵敏的辐射生物学指标之一
Objective To investigate the effect of low dose 147ppm contamination on hereditary mutation of erythrocyte and lymphocyte. Methods The bone marrow and blood smears of polychromatic and normal red blood cells micronucleus test and bone marrow lymphocyte chromosome aberration analysis. Results Compared with the normal control group, the intra-contaminant of 147kp (18.5kBq g) caused a significant increase of erythrocyte micronuclei rate and lymphocyte chromosome aberration rate (P <0.05 or 0.01). However, pre-application of contaminated animals at 147, 737 and 37Bq g / g, and infiltration of 147 pM at the level of 18 5kBq g after 3 days resulted in a significantly lower rate of erythrocyte micronuclei and lymphocyte chromosome aberration (P <0.05 or 0.01), and there was no significant difference in some cell mutations compared with the control group (P> 0.05). Conclusion Pretreatment with low dose of 147ppm could induce the radiation response of erythrocytes and lymphocytes for a long period of time. The cell-adapted response induced by contamination within 147ppm had a wide dose range. Distortion comparison, addicted to multiple staining and normal red blood cell micronucleus is also one of the more sensitive biological indicators of radiation