论文部分内容阅读
从山东地区产的成年鸭中分离出鸭乙肝病毒,人工感染当地1日龄雏麻鸭。用斑点酶法、斑点杂交法及用电子显微镜观察外周血中被感染鸭乙肝病毒不同时期的动态,并对不同感染阶段的肝组织病理变化进行了分析。结果表明,感染3周时鸭体内病毒复制较为活跃,此时肝组织病理损害也较严重。本实验为研究人类乙肝病毒的早期感染尤其是母婴传播过程外周血中乙肝病毒的动态与肝组织病理变化的关系提供了动物模型,为防治乙肝病毒早期感染提供了实验基础。
Duck hepatitis B virus was isolated from adult ducks produced in Shandong Province and artificial inoculation of 1-day-old brood duck was performed. The dynamic changes of different stages of infected duck hepatitis B virus in peripheral blood were observed by dot blot, dot blot hybridization and electron microscopy, and the pathological changes of liver tissues at different stages of infection were analyzed. The results showed that the virus replication in ducks was more active at 3 weeks after infection, and the pathological damage of liver tissue was more severe at this time. This experiment provided an animal model for studying the relationship between the early infection of human hepatitis B virus, especially the relationship between the dynamics of hepatitis B virus in the peripheral blood and the pathological changes of liver tissue during mother-to-child transmission, and provided the experimental basis for the prevention and treatment of hepatitis B virus early infection.