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目的 :研究ClC - 3Cl-通道在培养的牛脑血管平滑肌细胞 (CSMC)Ca2 + 池操纵性Ca2 + 内流中的作用。方法 :采用培养的CSMC ,用硫代磷酸修饰的ClC - 3反义寡核苷酸与脂质体一起转染入平滑肌细胞 4 8h ,以正义和随机序列寡核苷酸作为对照 ,在生物荧光双波长影像分析系统用Fura- 2
AIM: To investigate the role of ClC - 3Cl - channel in Ca2 + pool influx in cultured bovine cerebrovascular smooth muscle cells (CSMCs). Methods: Cultured CSMCs were transfected with smooth muscle cells by liposome with ClP - 3 antisense oligonucleotides modified with phosphorothioate for 48 h. Using sense and random oligonucleotides as control, Fura-2 for dual-wavelength image analysis system