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This research was to investigate physiological activities and the expression of insecticidal protein in cotton leaves of Kemian 1,a widely grown cotton cultivar in the lower reaches of the Yangtze River,treated under high (40℃ and 35℃) and low (25℃ and 20℃) temperatures for 48 h.The main results indicated that the contents of free amino acid and malondialdehyde (MDA) increased during the heat and cold stress with the biggest increments in the first 12 h;and the content of soluble protein,activities of superoxide dismutase (SOD),and glutamic-pyruvic transaminase (GPT)decreased during the stress with the biggest decrements also in the first 12 h;the activity of nitrate reductase (NR) decreased in the first 24 h,and during the remaining 24 h its activity followed a gradual uptrend.The content of insecticidal protein in cotton leaves followed a downtrend during the stress,and the biggest decrements occurred in the first 12 h,suggesting that the expression of insecticidal protein was immediately inhibited during the process of stress.The results also indicated that physiological activities and the expression of insecticidal protein in cotton leaves were closely related.Compared with other stress temperatures,40℃ produced more immediate effects on the physiological activities and insecticidal protein content.In contrast to the peak flowering period,the physiological activities and insecticidal protein expression at the peak boll-setting period were more susceptible to heat and cold stress.