论文部分内容阅读
目的 :探讨 p5 3基因在胃癌的表达与临床、病理因素及预后的关系 ,探索 p5 3基因异常表达在胃癌发生过程中的作用。方法 :使用抗p5 3蛋白的单克隆抗体DO - 7,对 98例人胃癌组织 p5 3的表达进行免疫组化研究。 结果 :48例胃癌组织 p5 3基因表达阳性 (49% )。p5 3基因表达与胃癌的大体类型、分化程度、生长方式、浸润深度、淋巴结转移等指标均无明显关系。在 10例有不典型增生的癌旁组织中 ,2例呈阳性反应 ;在 4例有肠上皮化生的癌旁组织中及其余正常的癌旁组织中均无阳性表达。根据有随访资料的 5 5例统计结果 ,显示 p5 3表达与胃癌预后无显著性相关 (P>0 .0 5 )。结论 :p5 3基因的突变可能是胃癌发生发展过程中的早期事件。由于 p5 3表达在胃癌组织有较高的阳性率 ,其测定可用于对高危人群的筛选。
Objective: To investigate the relationship between p53 gene expression and clinical, pathological factors and prognosis, and to explore the role of p53 gene aberrant expression in gastric carcinogenesis. Methods : Immunohistochemical study of the expression of p53 in 98 cases of human gastric cancer was performed using monoclonal antibody DO-7 against p53 protein. Results: The expression of p53 gene was positive in 49 cases of gastric cancer (49%). The expression of p53 gene was not significantly related to the general type, differentiation, growth pattern, depth of invasion, lymph node metastasis, and other indicators of gastric cancer. In 10 paracancerous tissues with atypical hyperplasia, 2 were positive; no positive expression was found in 4 paracancerous tissues with intestinal metaplasia and other normal paracancerous tissues. According to the statistical results of 55 cases with follow-up data, it was shown that the expression of p53 was not significantly associated with the prognosis of gastric cancer (P>0.05). Conclusion: The mutation of p53 gene may be an early event in the development of gastric cancer. Since the expression of p53 has a high positive rate in gastric cancer, its determination can be used to screen high-risk groups.