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The present study establishes the in vitro system for studying the oncosuppression activity of par-vovirus and discovers that parvovirus H-I can be grown cytolytically in various human cancer cell lines,which include 4 hepatoma cell lines (QGY--7703, SMMC--7721, Bel--7402, PLC/PRF/5), 3 gastric can-cer cell lines (SGC-7901, MGC-80-3, MKN-28) and 1 naspharynx cancer cell line (CNE). The growth oftwo primary gastric cancer cell cultures from surgical cancer tissue were also inhibited by the infectionof H-I. The sensitivity of cancer cells to H-I may relate to their differentiation states. On the con-trary, H-I can neither be grown cytolytically in normal liver or stomach cells, nor inhibit their growth.Transformation of human skin fibroblasts with Simian Virus 40 activated their sensitivity to H-I. Ourresults thus indicate that the antineoplastic activity of H-I in vivo involves at least its direct inhibit-ing or killing malignant cells.
The present study establishes the in vitro system for studying the oncosuppression activity of par-vovirus and discovers that parvovirus HI can be grown cytolytically in various human cancer cell lines, which include 4 hepatoma cell lines (QGY-7703, SMMC-7721, The growth of primary gastric cancer (Bel-7402, PLC / PRF / 5), 3 gastric can-cer cell lines (SGC-7901, MGC-80-3, MKN- 28) and 1 naspharynx cancer cell line Cell cultures from surgical cancer tissue were also inhibited by the infection of HI. The sensitivity of cancer cells to HI may relate to their differentiation states. On the con-trary, HI can neither be be grown cytolytically in normal liver or stomach cells, nor inhibit their growth. Transformation of human skin fibroblasts with Simian Virus 40 activated their sensitivity to HI. Our results thereby indicate that the antineoplastic activity of HI in vivo involved at least its direct inhibit-ing or killing malignant cells.