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目的：本研究通过比较研究ＨＣＣ 癌组织和癌周组织中ｐ５３ 基因密码子２４９ 特征性突变来进一步阐明该基因在ＨＣＣ 发生机制中的意义。方法：收集９ 份癌组织中经单链构型多态性分析研究证实存在密码子２４９ 突变的ＨＣＣ 标本和１１ 例无此突变的ＨＣＣ 标本，采用等位基因特异性ＰＣＲ（ＡＳ－ ＰＣＲ） 方法比较研究癌组织和癌周组织中密码子２４９ 突变情况。结果：２０ 例ＨＣＣ 中，癌周组织中密码子２４９ 特征性突变检出率为７０ ％ 。９ 例ＳＳＣＰ 阳性者的癌周组织中的特征性突变检出率达１００ ％ 。１１ 例阴性患者的癌组织和癌周组织也能检出一定水平的突变。结论：ｐ５３基因密码子２４９ 突变在非癌肝组织中广泛存在。癌组织中密码了２４９ 特征性突变可能发生在肿瘤形成之前。
OBJECTIVE: This study was to further elucidate the significance of this gene in the pathogenesis of HCC by comparing the characteristic mutations of the p53 gene codon 249 in HCC cancer tissues and pericancerous tissues. METHODS: A total of 9 HCC specimens with codon 249 mutations and 11 HCC specimens without this mutation were confirmed in 9 cancer tissues by single strand conformation polymorphism analysis. Allele-specific PCR (AS-PCR) was used. Comparative study of codon 249 mutations in cancer tissues and cancerous tissues. RESULTS: In 20 cases of HCC, the detection rate of the characteristic mutation of codon 249 in the cancerous tissue was 70%. The detection rate of characteristic mutations in the cancerous tissues of 9 SSCP-positive individuals was 100%. A negative level of mutation was also detected in the cancer tissues and the pericancerous tissues of 11 negative patients. Conclusion: The 249 mutation of p53 gene codon is widely found in non-cancerous liver tissue. A 249-character mutation in the cancerous tissue may precede the development of the tumor.