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目的探讨小鼠树突状细胞(DC)表面白细胞免疫球蛋白样受体2(LILRB2)改变对DC的CD11c、CD80和CD86表型的影响及意义。方法应用20 ng/ml重组小鼠粒细胞集落刺激因子(r GM-CSF)+20 ng/ml IL-4刺激小鼠骨髓细胞生长,隔日进行细胞换液,第5天实验组转染LILRB2受体干扰RNA,空白对照组转染空质粒,对照组单纯换液。培养过程中观察细胞形态学变化,并于转染72 h后,光镜下观察DC形态,用流式细胞仪测定细胞表面CD11c、CD80和CD86分子表达。结果光镜下对照组与实验组均可见DC细胞形态,流式细胞仪测定实验组CD11c(0.662±0.174)(n=12)与空白对照组CD11c(0.675±0.237)和对照组(0.688±0.076)分子表达无明显差异(P>0.05)。实验组DC细胞表面CD80(0.626±0.060)较空白对照组CD80(0.406±0.163)(n=12)和对照组CD80(0.409±0.100)表达均增加(P<0.05)。实验组DC细胞表面CD86(0.730±0.102)较空白对照组CD86(0.497±0.278)和对照组CD86(0.368±0.073)表达均增加(P<0.05)。结论应用LILRB2受体干扰RNA不影响DC细胞表面CD11c分子表达,而增加细胞表面CD80和CD86分子表达。细胞表面CD80和CD86分子作为免疫反应的重要协同刺激分子在抗原提呈细胞表面表达升高,则激活T细胞免疫反应的能力增强。
Objective To investigate the effect of dendritic cells (DCs) leukocyte immunoglobulin-like receptor 2 (LILRB2) on the phenotypes of CD11c, CD80 and CD86 in DCs. Methods The mouse bone marrow cells were stimulated with 20 ng / ml r GM-CSF and 20 ng / ml IL-4, and the cells were transplanted every other day. On the fifth day, the mice were transfused with LILRB2 Body interfering RNA, blank control group transfected with empty plasmid, the control group simply liquid change. Morphological changes were observed during the culture. After 72 h of transfection, the morphology of DCs was observed under light microscope. The expression of CD11c, CD80 and CD86 on the cell surface was measured by flow cytometry. Results The morphological features of DCs were observed in the control group and the experimental group under light microscope. The levels of CD11c (0.662 ± 0.174) (n = 12) and CD11c (0.675 ± 0.237) and control group (0.688 ± 0.076 ) Molecule expression no significant difference (P> 0.05). The expression of CD80 (0.626 ± 0.060) on the surface of DCs in the experimental group was significantly higher than that in the blank control group (0.806 ± 0.163) (n = 12) and the control group (0.409 ± 0.100) (P <0.05). The expression of CD86 (0.730 ± 0.102) in DCs in experimental group was significantly higher than that in control group (0.497 ± 0.278) and CD86 (0.368 ± 0.073) in control group (P <0.05). Conclusion Interfering RNA with LILRB2 receptor did not affect the expression of CD11c on the surface of DCs, but increased the expression of CD80 and CD86 on the surface of DCs. The cell surface CD80 and CD86 molecules as an important immune response co-stimulatory molecules in the antigen-presenting cell surface expression increased, the ability to activate T cell immune response enhanced.