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PAS作为一种特殊染色技术,可以清楚显示糖原、中性黏多糖、肾小球基底膜系膜基质、真菌孢子或菌丝等物质,广泛应用于人体和实验动物组织的诊断和研究,甚至有时可以替代HE染色[1]。但PAS反应中的Schiff试剂通常难以配制,作者对传统的Schiff试剂配制方法作了一些改进,效果良好,现介绍如下。1材料与方法1.1材料碱性品红、蒸馏水、盐酸、偏重亚硫酸钠、活
As a special staining technique, PAS can clearly display glycogen, neutral mucopolysaccharide, glomerular basement membrane mesangial matrix, fungal spores or mycelium and other substances, which are widely used in the diagnosis and research of human and experimental animal tissues, and even Sometimes can replace HE staining [1]. However, the Schiff reagent in the PAS reaction is usually difficult to formulate. The author made some improvements to the traditional Schiff reagent preparation method, and the effect is good as follows. 1 Materials and Methods 1.1 Materials Basic Fuchsin, distilled water, hydrochloric acid, sodium metabisulfite, live