论文部分内容阅读
选用Epstin-Barr病毒(EBV)基因组内部重复序列1(IR1)片断作为多聚酶链反应(Polymerase Chain Reaction,PCR)扩增引物,用于检测了31例不同病例活检组织和4例新鲜鼻咽组织经体外培养6周以上的新生上皮细胞内EBV基因,其中检出EBVDNA:高分化鼻咽癌5/5,低分化鼻咽癌4/4,何杰金氏病5/5,非何杰金氏病0/2,头颈其他肿瘤1/6,鼻咽慢性炎症0/5,正常鼻咽组织0/4;新生上皮细胞DNA抽提物;低分化鼻咽癌2/2,炎症0/1,正常人胚鼻咽上皮0/1;携带EBV基因组细胞系(Raji,B_(95-8)各1)2/2,致淋巴细胞转化之B_(95-8)病毒为10~(-4),PCR检测10~(-4)～10~(-6)均阳性,10~(-7)未检出。结果表明EBV与鼻咽癌与何杰金氏病有关,常规石蜡包埋切片仅8μm×0.1mm~2,贮存时间至三年仍可用于PCR检测EBV DNA,证实PCR是一种快速、灵敏和特异测捡EBV基因组的方法,可作为肿瘤和疚病病毒病因回顾性调直研究的有力手段。
Epitin-Barr virus (EBV) internal repeat 1 (IR1) fragment was selected as a polymerase chain reaction (PCR) amplification primer for the detection of 31 cases of different cases of biopsy and 4 cases of fresh nasopharyngeal tissue In vitro cultured EBV genes in neonatal epithelial cells for more than 6 weeks, including EBV DNA: 5/5 highly differentiated nasopharyngeal carcinoma, 4/4 poorly differentiated nasopharyngeal carcinoma, 5/5 Hodgkin’s disease, non-Hodgkin’s disease Disease 0/2, other head and neck tumors 1/6, chronic inflammation of the nasopharynx 0/5, normal nasopharyngeal tissue 0/4; nascent epithelial DNA extract; poorly differentiated nasopharyngeal carcinoma 2/2, inflammation 0/1, Normal human embryonic nasopharyngeal epithelial 0/1; Bv (95-8) induced by lymphocyte transformation was 10 -4 (-4) cells carrying EBV genomic cell line (Raji, B_ (95-8) The positive rates of 10 ~ (-4) ~ 10 ~ (-6) were detected by PCR, but not detected by 10 ~ (-7). The results showed that EBV was associated with Hodgkin’s disease and nasopharyngeal carcinoma. The conventional paraffin-embedded sections were only 8μm × 0.1mm ~ 2. The EBV DNA could still be used to detect the EBV DNA after storage for three years, confirming that PCR is a rapid, sensitive and The specific detection of EBV genome can be used as a powerful retrospective study of the etiology of cancer and guilty virus.