论文部分内容阅读
目的:探讨人非小细胞肺癌(non-small cell lung cancer,NSCLC)患者中缺氧诱导因子-1α(Hypoxia inducible factor-1α,HIF-1α)的表达及临床意义。方法:采用免疫组化染色方法检测80例NSCLC及12例正常肺组织标本中HIF-1α、Ki-67和血管内皮生长因子(Vascular Endothelial Growth Factor,VEGF)的表达水平,分析其表达与肿瘤细胞增殖、血管新生及预后的关系。结果:NSCLC组织中HIF-1α表达率(63.75%)显著高于正常肺组织(25.00%)(P<0.05),NSCLC组织中HIF-1α表达水平与肿瘤大小、分化程度、淋巴结转移及TMN分期密切相关(P<0.05),在HIF-1α高表达组,相应Ki-67、VEGF的阳性表达率增高(P<0.05)。Kaplan-Meier单因素生存分析显示HIF-1α高表达为NSCLC患者预后的不良因素(P<0.05),COX多因素回归分析同样显示HIF-1α高表达为NSCLC患者不良预后的独立因素。结论:HIF-1α高表达可促进NSCLC患者肿瘤细胞增殖并诱导新生血管形成,是肿瘤恶性程度及疾病预后的重要检测标志物。
Objective: To investigate the expression and clinical significance of hypoxia inducible factor-1α (HIF-1α) in patients with non-small cell lung cancer (NSCLC). Methods: The expressions of HIF-1α, Ki-67 and Vascular Endothelial Growth Factor (VEGF) in 80 specimens of NSCLC and 12 specimens of normal lung tissue were detected by immunohistochemical staining. The expression of HIF-1α, Ki- Proliferation, Angiogenesis and Prognosis. Results: The positive expression rate of HIF-1α in NSCLC tissues was significantly higher than that in normal lung tissues (63.75%, 25.00%) (P <0.05). The expression of HIF-1α in NSCLC tissues was correlated with tumor size, differentiation degree, lymph node metastasis and TMN stage (P <0.05). The positive rates of Ki-67 and VEGF in HIF-1α overexpression group were significantly higher than those in control group (P <0.05). Kaplan-Meier univariate survival analysis showed that HIF-1α overexpression was a poor prognostic factor in patients with NSCLC (P <0.05). COX multivariate regression analysis also showed that HIF-1α overexpression was an independent factor of poor prognosis in patients with NSCLC. Conclusion: High expression of HIF-1α can promote tumor cell proliferation and induce neovascularization in NSCLC patients. It is an important marker of tumor malignancy and disease prognosis.