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目的制备多功能金壳表面修饰的磁性纳米粒,考察其形貌、粒径、双光子光学信号、细胞摄取、细胞毒性及释药特性。方法采用乳化溶剂蒸发法联合羟胺还原法制备金壳包裹的磁性聚酯纳米粒;利用动态光散射法测定其粒径;原子力显微镜及透射电镜考察其形貌;多光子共聚焦显微镜考察其双光子光学特性。以伊达比星为模型药物,采用透析法考察其体外释药特性。结果制备所得的纳米粒子外观呈球形或类球形,平均粒径为(235.7±1.34)nm,包封率为72.08%,载药率为1.73%,在近红外光(800 nm)激发下,金壳的双光子发射光谱具有指纹特征性。体外释药研究表明,药物的释放是可受外界激光辐射控制的。结论成功制备了多功能金壳包裹的磁性聚酯纳米粒。通过在多聚物纳米粒子外层覆盖一层金壳可实现在飞秒激光激发下的自显影和控制药物精准释放的双重功能,是一种新颖的纳米药物输送策略。
Objective To prepare multifunctional magnetic nanoparticles modified with gold shell surface and study their morphology, particle size, two-photon optical signal, cell uptake, cytotoxicity and drug release characteristics. Methods Magnetic gold nanoparticles encapsulated in gold shell were prepared by emulsifying solvent evaporation combined with hydroxylamine reduction method. The particle size was determined by dynamic light scattering method. Morphology was observed by atomic force microscopy and transmission electron microscopy. Two-photon confocal microscopy Optical characteristics. Idarubicin as a model drug, dialysis was used to investigate its in vitro release characteristics. Results The prepared nanoparticles showed a spherical or spheroidal shape with an average diameter of (235.7 ± 1.34) nm, encapsulation efficiency of 72.08% and drug loading rate of 1.73%. Under the excitation of near infrared (800 nm) The two-photon emission spectrum of the shell has fingerprint characteristics. In vitro release studies have shown that drug release is controlled by external laser radiation. Conclusion Multifunctional gold-coated magnetic polyester nanoparticles were successfully prepared. By covering the outer layer of polymer nanoparticles with a gold shell, dual functions of self-imaging and controlled release of drugs under femtosecond laser excitation can be achieved, which is a novel drug delivery strategy.