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目的:研究宫内缺氧的时间对胎鼠神经干细胞增殖的影响,以便对临床上缺血缺氧性脑病的诊断及治疗提供理论基础。方法:通过制作不同时间段的孕鼠缺氧模型,然后脱颈处死孕鼠,暴露部分脑组织后放入10%多聚甲醛溶液中固定,取海马与齿状回互包平面制片备用,再进行免疫组化染色、显微镜下观察和照相,分析图片。结果:缺氧时间愈长则阳性细胞染色减弱,但染色除缺氧12 h组以外,其余各缺氧组阳性细胞染色较对照组增强,数量增多,提示适量的缺氧神经细胞有刺激性的生长;随着时间的延长,在12 h后神经细胞的增速生长表现为抑制。结论:缺氧时程12 h以下神经细胞的增速生长表现为活跃,反之抑制。
Objective: To study the effect of intrauterine hypoxia on the proliferation of fetal neural stem cells in order to provide a theoretical basis for the diagnosis and treatment of hypoxic ischemic encephalopathy. Methods: Pregnant mice were sacrificed by making hypoxia model of pregnant rats at different time periods, and then the pregnant rats were killed by atrophy. After exposure to some brain tissues, the rats were placed in 10% paraformaldehyde solution and fixed. Further immunohistochemical staining, microscopic observation and photography, analysis of the picture. Results: The longer the hypoxia time, the staining of the positive cells was weakened. However, in addition to the hypoxia for 12 h, the staining of positive cells in other hypoxia groups was stronger than that in the control group, indicating that the appropriate amount of hypoxic neurons were irritating Growth; with the extension of time, after 12 h growth of nerve cells showed growth inhibition. CONCLUSION: The growth of neurons under hypoxia for 12 hours is obviously active and vice versa.