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目的:探讨溃疡性结肠炎（UC）患者结肠组织中维生素D受体（VDR）表达水平与Th17/调节性T细胞（Treg）比例失衡的关系。方法:回顾性收集30例UC患者和15例结肠腺瘤对照者的外周血和结肠组织标本，同时记录上述研究对象的临床资料。采用电化学发光法检测血清25-羟维生素D[25（OH）D]水平；采用免疫组化检测结肠组织中VDR、Th17细胞和Treg细胞相关因子[RORγt、白介素（IL）-17、Foxp3、IL-10]的表达水平。结果:UC患者结肠组织中RORγt、IL-17、Foxp3、IL-10表达水平及RORγt/Foxp3比值均高于对照组[0.32（0.20，0.46）×10n -2比0.17（0.11，0.19）×10n -2，0.56（0.30，0.76）×10n -2比0.19（0.16，0.26）×10n -2，0.26（0.16，0.33）×10n -2比0.18（0.12，0.21）×10n -2，0.29（0.19，0.37）×10n -2比0.06（0.04，0.09）×10n -2，1.14（0.81，1.86）比0.86（0.78，0.92），n P均<0.05]，并且活动期患者高于缓解期患者[0.45（0.31，0.53）×10n -2比0.16（0.13，0.20）×10n -2，0.73（0.56，0.78）×10n -2比0.26（0.21，0.33）×10n -2，0.26（0.24，0.35）×10n -2比0.17（0.14，0.26）×10n -2，0.35（0.28，0.38）×10n -2比0.16（0.11，0.20）×10n -2，1.77（0.90，2.07）比82.84（64.12，112.67），n P均<0.05]。UC患者结肠组织中VDR表达水平显著低于对照组[0.66（0.28，19.15）×10n -2比2.94（2.61，3.21）×10n -2，n P<0.001]，并且活动期患者低于缓解期患者[0.37（0.26，0.69）×10n -2比2.63（1.78，3.24）×10n -2，n P<0.001]。线性回归分析发现UC患者结肠组织中RORγt/Foxp3比值与VDR表达水平成独立负相关（n β = -0.530，n P = 0.003）。n 结论:UC患者结肠组织中VDR表达降低与Th17/Treg细胞失衡相关。“,”Objects:To explore the association of colonic vitamin D receptor (VDR) expressionwith imbalance of Th17/regulatory T cells (Tregs) in patients with ulcerative colitis (UC) .Methods:The specimens of peripheral blood and colonic tissue were retrospectively collected from 30 UC patients and 15 controls (colonic polypadenoma) , respectively. The clinical data of all the participants were simultaneously recorded, including age, gender, smoking, body mass index, UC activity (assessed by Mayo scoring system) . The electrochemiluminescence method was conducted to measure the serum level of 25-hydroxyvitamin D [25 (OH) D]. Moreover, the immunohistochemical staining method was applied for examining the protein expression levels of VDR, Th17 and Treg cells related factors (RORγt, IL-17, Foxp3, IL-10, respectively) in the colonic tissues.Results:Compared with the controls, the expressions of RORγt, IL-17, Foxp3 and IL-10, together with the ratio of RORγt/Foxp3, were significantly higher in UC patients [0.32 (0.20, 0.46) ×10n -2 vs. 0.17 (0.11, 0.19) ×10n -2, 0.56 (0.30, 0.76) ×10n -2 vs. 0.19 (0.16, 0.26) ×10n -2, 0.26 (0.16, 0.33) ×10n -2 vs. 0.18 (0.12, 0.21) ×10n -2, 0.29 (0.19, 0.37) ×10n -2 vs. 0.06 (0.04, 0.09) ×10n -2, 1.14 (0.81, 1.86) vs. 0.86 (0.78, 0.92) , all n P<0.05], meanwhile the expressions of RORγt, IL-17, Foxp3, IL-10, and the ratio of RORγt/Foxp3 were higher in patients with active UC than inactive UC [0.45 (0.31, 0.53) ×10n -2 vs. 0.16 (0.13, 0.20) ×10n -2, 0.73 (0.56, 0.78) ×10n -2 vs. 0.26 (0.21, 0.33) ×10n -2, 0.26 (0.24, 0.35) ×10n -2 vs. 0.17 (0.14, 0.26) ×10n -2, 0.35 (0.28, 0.38) ×10n -2 vs. 0.16 (0.11, 0.20) ×10n -2, 1.77 (0.90, 2.07) vs. 82.84 (64.12, 112.67) , all n P<0.05]. The colonic VDR expression in UC patients was significantly lower than that in controls [0.66 (0.28, 19.15) ×10n -2 vs. 2.94 (2.61, 3.21) ×10n -2, n P<0.001], meanwhile VDR expression was also shown to be lower in patients with active UC than that in those with inactive UC [0.37 (0.26, 0.69) ×10n -2 vs. 2.63 (1.78, 3.24) ×10n -2, n P<0.001]. The subsequent linear regression analysis suggested that the ratio of RORγt/Foxp3 in colonic tissue of UC patients was independently and negatively associated to the colonic VDR expression (n β = -0.530, n P = 0.003) .n Conclusion:The lower colonic VDR expression is associated to the imbalance of Th17/Treg cells in UC patients.