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为探讨邻苯二甲酸二(2-乙基己基)酯(di-2-ethylhexyl phthalate,DEHP)对两栖动物精巢类固醇激素合成的影响,将中国林蛙(Rana chensinensis)雄性成体分别暴露于浓度为10-7、10-6、10-5、10-4mol·L-1DEHP的水体,分别在暴露20、30和40d取其精巢,提取精巢总RNA,逆转录合成cDNA,通过荧光实时定量PCR检测StAR、CYP17和CYP19mRNA表达相对值。结果表明:与对照组相比,DEHP处理组StAR和CYP19基因表达均上调,CYP17基因表达下调;比较不同DEHP浓度和不同暴露时间对StAR、CYP17和CYP19mRNA表达相对值的影响,显示DEHP浓度变化对3个基因表达影响的规律性不强,而DEHP暴露时间的累积效应较明显;提示DEHP可通过干扰中国林蛙精巢中StAR、CYP17和CYP19基因表达,影响其相应关键酶的表达,从而干扰类固醇激素的合成,产生雌激素效应。
To investigate the effect of di-2-ethylhexyl phthalate (DEHP) on the synthesis of testosterone in amphibians, male Rana chensinensis adults were exposed to a concentration of 10-7, 10-6, 10-5, 10-4 mol·L-1 DEHP water respectively. The testis was collected at 20, 30, 40 days after exposure. Total RNA was extracted from the testis and cDNA was reverse transcribed. The fluorescence quantitative real- Relative values of StAR, CYP17 and CYP19 mRNA expression. The results showed that compared with the control group, the expression of StAR and CYP19 genes were up-regulated and the expression of CYP17 gene was down-regulated in DEHP-treated group. The relative expression of StAR, CYP17 and CYP19 mRNA in different concentrations of DEHP and different exposure time were compared. The regularity of the influence of three gene expression was not strong, while the cumulative effect of DEHP exposure time was more obvious; suggesting that DEHP can interfere with the expression of StAR, CYP17 and CYP19 gene in the testis of Chinese Rana chensinensis, affecting the expression of corresponding key enzymes and interfering steroid Hormone synthesis, resulting in estrogen effects.