论文部分内容阅读
目的 :探讨血清羟脯氨酸浓度和脯肽酶活性在反映肾纤维化中的意义。方法 :经肾活检证实的有(纤维化组)和无(无纤维化组)肾小球硬化与肾间质纤维化的肾脏病人各10例 ,健康献血员(对照组)10例 ,测血清羟脯氨酸浓度、血清脯肽酶活性等指标。结果 :纤维化组血清羟脯氨酸浓度显著高于无纤维化组(P<0.001)和健康对照组(P<0.001) ,以此指标显著增高判断肾纤维化 ,敏感性100 % ,特异性91 % ,假阳性9 % ,与肾活检符合率95 %。纤维化组血清脯肽酶活性亦显著高于无纤维化组(P<0.05)和健康对照组(P<0.05) ,以此指标显著增高判断肾纤维化 ,敏感性80 % ,特异性100 % ,未发现假阳性 ,与肾活检符合率90 %。血清羟脯氨酸浓度与患者血清肌酐浓度、纤维化肾小球比率和病变肾小管、肾间质比率显著正相关。结论 :血清羟脯氨酸浓度和脯肽酶活性 ,可作为判断肾脏病人肾纤维化的无创性检查指标 ,尤其适用于有肾活检禁忌症的病人。
Objective: To investigate the significance of serum hydroxyproline concentration and procollagenase activity in reflecting renal fibrosis. Methods: 10 cases of renal patients with (fibrosis group) and no (no fibrosis group) glomerulosclerosis and interstitial fibrosis were confirmed by renal biopsy, 10 healthy donors (control group), serum Hydroxyproline concentration, serum prolidinase activity and other indicators. Results: Serum hydroxyproline concentration in fibrosis group was significantly higher than that in non-fibrosis group (P <0.001) and healthy control group (P <0.001) 91%, false positive 9%, and 95% compliance with the renal biopsy. Serum procollagenase activity in fibrosis group was also significantly higher than that in non-fibrosis group (P <0.05) and healthy control group (P <0.05), and the index was significantly higher in predicting renal fibrosis with a sensitivity of 80% and a specificity of 100% , Did not find false positive, and the biopsy coincidence rate of 90%. Serum hydroxyproline concentration and serum creatinine levels, fibrosis glomerular ratio and pathological renal tubules, renal interstitial ratio was significantly positively correlated. Conclusion: Serum hydroxyproline concentration and procollagenase activity can be used as a noninvasive index for judging kidney fibrosis in patients with kidney disease, especially for patients with contraindications to renal biopsy.