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目的 :了解儿童哮喘α1 抗糜蛋白酶(α1 antichymotrypsin,α1 ACT)基因点突变情况。方法 :应用聚合酶链反应(PCR)和限制性酶切图谱分析技术 ,对100例重庆市汉族儿童哮喘α1 ACT基因外显子(exon)Ⅱ和Ⅲ进行检测。结果 :所有研究对象均未发现Bonn 1及Bochum 1变异体。结论 :Bonn 1和Bochum 1变异体与100例重庆市汉族儿童哮喘发病无平行关系。
Objective: To understand the point mutation of α1 antichymotrypsin (α1 ACT) gene in childhood asthma. Methods: The exon Ⅱ and Ⅲ of α1 ACT gene in 100 children with asthma were detected by polymerase chain reaction (PCR) and restriction analysis. RESULTS: Bonn 1 and Bochum 1 variants were not found in all subjects. Conclusion: There is no parallel relationship between Bonn 1 and Bochum 1 variants and the incidence of asthma in 100 Han children in Chongqing.