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目的 :对脑内兴奋性氨基酸的变化进行定位研究。方法 :应用Beckman 6300氨基酸分析仪和601黄金系统色谱工作站 ,对梭曼惊厥后不同时相大鼠的新鲜脑组织进行定位检测。结果 :梭曼诱发惊厥后脑内谷氨酸和天门冬氨酸的水平显著降低 ,谷氨酸下降最明显的是惊厥30min后的大脑皮质和海马 ,分别是正常组的53.2 %和52.6 % ,小脑的嗅球内谷氨酸水平显著下降 ,天门冬氨酸更易受梭曼中毒的影响 ,惊厥后5、30、90min3个时点测定的6个脑区(纹状体、大脑皮质、海马、中脑、小脑、间脑和嗅球)中均显著下降。结论 :梭曼中毒后脑内谷氨酸和天门冬氨酸水平显著降低 ,递质耗竭是递质活动增加的征象 ,因此 ,谷氨酸和天门冬氨酸递质活动增加可能在梭曼中毒性惊厥的发生和维持中起重要作用。
Objective: To study the changes of excitatory amino acids in brain. Methods: Beckman 6300 amino acid analyzer and 601 gold system chromatography workstation were used to detect the location of fresh brain tissue in rats of different phases after soman seizure. Results: The levels of glutamate and aspartate in the brain were significantly decreased after soman-induced seizures. The most significant reduction of glutamate was cerebral cortex and hippocampus 30min after seizures, which were 53.2% and 52.6% in the normal group, Of the olfactory bulb glutamate levels decreased significantly, aspartic acid more susceptible to soman intoxication, seizures at 5,30,90 min 3 time points measured 6 brain regions (striatum, cerebral cortex, hippocampus, midbrain , Cerebellum, diencephalon and olfactory bulb) were significantly decreased. CONCLUSIONS: The levels of glutamate and aspartate in the brain are significantly decreased after soman poisoning, and neurotransmitter depletion is a sign of increased neurotransmitter activity. Therefore, increased activities of neurotransmitter glutamate and aspartate may be associated with soman toxicity Occurrence and maintenance of convulsions play an important role.