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研究证明:许多化学药物的治疗都是以诱导细胞凋亡的方式杀伤癌细胞的。有关细胞凋亡的研究已成为肿瘤生物学研究中的一个新焦点。IgYricinA为本实验室新研制的一种免疫毒。与其它种类的免疫毒一样,它能够特异性地杀伤靶细胞。推测:免疫毒IgYricinA也是以诱导细胞凋亡的方式杀死癌细胞的。我们已经研究了免疫毒对靶细胞的杀伤能力和效果,本文则着重对细胞的杀伤机理进行探讨。本实验以胃癌细胞MGC803为靶细胞,HeLa细胞为非特异识别细胞,二倍体2BS细胞为正常对照细胞,进行IgYricinA处理,观察其诱导细胞凋亡的可能性。同时进行IgY和ricin处理,以期探明引起细胞凋亡的有效成分。OlympusBX荧光显微镜下观察到(Fig2,5&6):在细胞培养液中加入IgYricinA后,MGC803细胞表现出典型的凋亡形态变化,即染色质浓缩、细胞皱缩并放出凋亡小体。细胞凋亡时DNA被降解成寡核体长度的片段,在琼脂凝胶电泳上形成DNA梯带,成为药物处理后确定细胞凋亡过程中细胞降解的一种生化指标。Fig3表明,用IgYricinA处理MGC803细胞24小时、用ricin处理MGC803和HeLa?
Research shows that many chemotherapeutic agents kill cancer cells in a way that induces apoptosis. Research on apoptosis has become a new focus of tumor biology research. IgY jamin A newly developed laboratory for an immunotoxicity. Like other kinds of immunotoxins, it is able to specifically kill target cells. Speculated that: Immunotoxicity IgY rubin also kill cancer cells in a way to induce apoptosis. We have studied the immunogenicity of target cells killing ability and effectiveness, this article focuses on the cell killing mechanism to explore. In this study, gastric cancer cells MGC 803 as target cells, HeLa cells as non-specific identification of cells, diploid 2BS cells as normal control cells, IgY rubin treatment, to observe the possibility of inducing apoptosis. At the same time IgY and ricin treatment, in order to identify the active ingredients that cause apoptosis. OlympusBX fluorescence microscope (Fig 2, 5 & 6): MGC 803 cells showed typical morphological changes of apoptosis, that is, chromatin condensation, cell shrinkage and release of apoptosis Death body. DNA is degraded into oligorigonucleotide fragments upon apoptosis, forming DNA ladder on agarose gel electrophoresis, becoming a biochemical indicator of cellular degradation during drug treatment after apoptosis. Fig3 shows that MGC803 cells treated with IgYricinA for 24 hours, treated with ricin MGC 803 and HeLa?