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目的:建立以RP-HPLC法测定人血浆中头孢他美浓度的方法。方法:色谱柱为Hypersil C18,流动相为0.04%高氯酸溶液-乙腈(83∶17),柱温为40℃,流速为1.0mL.min-1,检测波长为265nm。结果:头孢他美检测浓度在0.125~8.0μg.mL-1范围内与峰面积线性关系良好(r=0.999 8),最低检出浓度为0.125μg.mL-1,平均回收率为100.6%(RSD=1.2%);日内RSD≤5.3%(n=5),日间RSD≤4.0%(n=5)。结论:本方法简便、灵敏、准确、重现性好,可为头孢他美酯胶囊药动学和生物利用度研究提供参考。
Objective: To establish a method for the determination of ceftazidime in human plasma by RP-HPLC. Methods: The chromatographic column was Hypersil C18. The mobile phase was 0.04% perchloric acid solution - acetonitrile (83:17). The column temperature was 40 ℃, the flow rate was 1.0mL.min-1 and the detection wavelength was 265nm. Results: The calibration curve of ceftazidime was linear with the peak area in the range of 0.125 ~ 8.0μg.mL-1 (r = 0.999 8) and the lowest detection limit was 0.125μg.mL-1 with the average recovery of 100.6% RSD = 1.2%); intraday RSD≤5.3% (n = 5), daytime RSD≤4.0% (n = 5). Conclusion: The method is simple, sensitive, accurate and reproducible. It can provide reference for the study of pharmacokinetics and bioavailability of ceftazidime capsule.