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从猴头菌子实体中分离得到一种新型的水溶性杂多糖HEPF2,分子量大小为1.66×104Da,该多糖由岩藻糖、半乳糖和葡萄糖以1.00:3.69:5.42比例构成,同时也含有微量的3-O-甲基鼠李糖。进一步利用傅立叶变换红外光谱法、糖组成分析、甲基化分析、部分酸水解法和核磁共振法等方法进行结构鉴定,检测结果表明,该杂多糖中包含1→4、1→6结合的葡萄糖和1→6结合的半乳糖残基,连接于主链的侧链残基,包括岩藻糖残基、少数的端基葡萄糖和半乳糖残基。核磁共振法检测结果还表明,1→4结合葡萄糖为β构型,(1→6)结合半乳糖、(1→2,6)结合半乳糖和端基葡萄糖均为α构型。
A novel water-soluble heteropolysaccharide HEPF2 was isolated from the fruiting body of Hericium erinaceus and its molecular weight was 1.66 × 104Da. The polysaccharide consisted of fucose, galactose and glucose in a ratio of 1.00: 3.69: 5.42, and also contained traces of 3-O-methyl rhamnose. The structures were further identified by Fourier transform infrared spectroscopy, sugar composition analysis, methylation analysis, partial acid hydrolysis and nuclear magnetic resonance. The results showed that the heteropolysaccharide contains 1 → 4, 1 → 6 bound glucose And 1 → 6 galactose residues, attached to the backbone of the side chain residues, including fucose residues, a small number of terminal glucose and galactose residues. The results of NMR also showed that 1 → 4 bound glucose to β configuration, (1 → 6) bound to galactose, (1 → 2,6) bound galactose and terminal glucose were α configuration.